Optimasi Ketahanan Benih Ikan Nila Salin (Oreochromis niloticus) Terhadap Infeksi Streptococcocis
DOI:
https://doi.org/10.31850/jgt.v10i1.764Keywords:
Streptococcus iniae, seed, disease, Relative Percent Survival, lysozymeAbstract
This study aims to determine the resistance level of saline tilapia (Oreochromis niloticus) seeds obtained from tilapia broodstock that has been vaccinated with attenuated S. iniae bacteria against streptococcocis infection. This research was conducted in June-September 2020 at the Mini Hatchery Laboratory of the Department of Fisheries, Faculty of Marine and Fisheries Sciences, Hasanuddin University. The fish seeds used are 3-5 cm in size and 7-10 g in weight. The bacteria Streptococcus iniae were soaked in seeds for 30 minutes using concentrations of 105 CFU/mL, 107CFU/mL, 109CFU/mL, and without immersion. The seeds were then kept in a 40x25x20cm3 aquarium with a density of 50 seeds per aquarium for one month and fed at satiation. During the experiment, the media quality of the seeds was maintained within an acceptable range for the growth and viability of saline tilapia. The parameters observed were survival, RPS, and lysozyme activity in saline tilapia seeds. The optimal result that can increase the resistance of saline tilapia fry against Streptococcocis infection is at a concentration of 107 CFU/mL. This concentration can increase the survival rate, RPS, and lysozyme activity in saline tilapia.
References
Abraham, T. J., Namdeo, M. S., Adikesavalu, H., & Banerjee, S. (2019). Pathogenicity and Pathology of Streptococcus Agalactiae in Challenged Mozambique Tilapia Oreochromis mossambicus (Peters 1852) Juveniles. Aquatic Research, 2(4), 182-190.
Aryati, Y., & Supriyadi, H. (2010). Eksplorasi Bakteri Probiotik Sebagai Antibakteri Untuk Penanggulangan Penyakit Streptococcosis. Pusat Riset Perikanan Budidaya, Jakarta Selatan.
Austin, B., & Austin, A. (1999). Bacterial Fish Pathogen. Desease of Formed anf wild Fish. John Wiley and Sons. New York. P 457.
Baiano, J. C., & Barnes, A. C. (2009). Towards control of Streptococcus iniae. Emerging infectious diseases, 15(12), 1891.
Ellis, A. E. (1990). Lysozyme assays. Techniques in fish immunology, 1, 101-103.
Engstad, R. E., Robertsen, B., & Frivold, E. (1992). Yeast glucan induces increase in lysozyme and complement-mediated haemolytic activity in Atlantic salmon blood. Fish & Shellfish Immunology, 2(4), 287-297.
Evans, J. J., Shoemaker, C. A., & Klesius, P. H. (2003). Effects of sublethal dissolved oxygen stress on blood glucose and susceptibility to Streptococcus agalactiae in Nile tilapia Oreochromis niloticus. Journal of Aquatic Animal Health, 15(3), 202-208.
Hanif, A., Bakopoulos, V., & Dimitriadis, G. J. (2004). Maternal transfer of humoral specific and non-specific immune parameters to sea bream (Sparus aurata) larvae. Fish & shellfish immunology, 17(5), 411-435.
Lusiastuti, A. M., Purwaningsih, U., & Sumiati, T. (2010). Isolasi bakteriofaga anti Streptococcus agalactiae dari ikan nila (Oreochromis niloticus). Jurnal Riset Akuakultur, 5(2), 237-243.
Nissa, K., Sukenda, S., Junior, M. Z., Lusiastuti, A. M., & Nuryati, S. (2016). Benih Keturunan Induk Ikan Nila yang Divaksinasi pada Tingkat Kematangan Gonad-2 Lebih Tahan Terhadap Infeksi Streptococcus agalactiae (Resistance of tilapia (Oreochrimis niloticus) fry vaccinated at different gonadal developmental stages toward streptoco. Jurnal Veteriner, 17(3), 355-364.
Nursyirwani, N., Asmara, W., Wahyuni, A. E. T. H., & Triyanto, T. (2011). Properti Probiotik Isolat Bakteri Asam Laktat untuk Mengendalikan Pertumbuhan Vibrio alginolyticus pada Ikan Kerapu Macan (Epinephelus fuscoguttatus). Ilmu Kelautan: Indonesian Journal of Marine Sciences, 16(3), 151-158.
Purwaningsih, U. (2010). Vaksin Anti Streptococcus spp, Inakivitas Melalui Heatkilled Untuk Pencegahan Penyakit Streptococcosis Pada Ikan Nila (Oreochorimis niloticus). Balai Riset Perikanan Budidaya Air Tawar. Bogor.
Saurabh, S., & Sahoo, P. K. (2008). Lysozyme: an important defence molecule of fish innate immune system. Aquaculture research, 39(3), 223-239.
Sukenda, R., Nuryati, S., & Hidayatullah, D. (2015). Durasi proteksi vaksin Streptococcus agalactiae untuk pencegahan streptococcosis pada ikan nila. Jurnal Akuakultur Indonesia, 14(2), 192-201.
Swain, P., & Nayak, S. K. (2009). Role of maternally derived immunity in fish. Fish & shellfish immunology, 27(2), 89-99. http://dx.doi.org/10.1016/j.fsi.2009.04.008.
Yanong, R. P., & Francis-Floyd, R. (2002). Streptococcal infections of fish. Florida Cooperative Extension Service. IFAS, University of Florida, 1-5.
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